The greenovation® Product and Service-Portfolio

Bryo-SpeedTM – The Prototyping-System

By means of moss-protoplasts in the framework of pilot studies, a customized, qualitatively highly valuable glycoprotein can be produced in low quantities within only four to six weeks and can be applied for first tests in the laboratory. This guarantees high flexibility for our customers and a fast implementation of their ideas.

Bryo-SpeedTM: Fast and cost-effective making of glycoproteins through moss protoplasts
At the beginning, moss protonema is cultivated in photobioreactors. The moss tissue is harvested and treated with special enzymes which digest the cell wall of the moss. Moss cells without cell wall emanate from this process – the so-called protoplasts. Next, the moss protoplasts are transfected with DNA (red) coding for the desired product. The transfected protoplasts are transferred to multiwell plates in which they are cultivated for the production of the therapeutic protein. The product is secreted from the protoplast into the supernatant. The supernatant is harvested and replaced by fresh medium. The protein of interest is purified from the collected supernatant fractions and analyzed.