GREENOVATION - Pharmaceutical Protein Production in Moss

DQS-Quality Management


1999, the scientific founders of greenovation,

GREENOVATION. Prof. Ralf Reski

Prof. Dr. Ralf Reski
and GREENOVATION, Prof. Neuhaus Guenter

Prof. Dr. Gunther Neuhaus,

decided to set up a company for molecular biothechnology in plants with an initial emphasis on Golden Rice,

Process Development in a 5 L Photo-Bioreactor

a variety of rice produced through genetic engineering to biosynthesize beta-carotene, a precursor of provitamin A in the edible parts of rice.

2001 the company decided to shift its research activities towards production of therapeutic proteins in Physcomitrella patens.

2002 production of proteins in moss was established in a fully contained and controlled photo-bioreactor system.

2003 the company successfully established a double-knock-out moss strain, eliminating potentially immunogenic 1,2-xylose and 1,3-fucose on its N-glycans. Antibodies, produced in this strain were free of these structures. Furthermore, they were proven to trigger cellular cytotoxicity much more efficiently due to a plant-inherent lack of core 1,6-fucose on their N-glycans.

2004 the production of complex proteins in 5L photo-bioreactors was consolidated. BryoSpeed™, the transient production of proteins in moss was established as a fast track process for protein production.

Photobioreactor 50L as disposable Wave® technology

2005 the BryoMaster™ process based on stable cell lines was consolidated and reduced to practice in 5L photo-bioreactors. Several glycotransferase knock-out strains were added to the portfolio.

2007 Conceptualization of scalable tubular photo-bioreactors in cooperation with Sartorius Stedim.

2008 Delivery of first modular 100L tubular photo-bioreactor. Parallel development in disposable rocker bag system.

2009 Successful transfer of 5L photo-bioreactor process into 100L tubular photo-bioreactor and 200L disposable bag system.