BryoTechnology

Moss Inherent

Physcomitrellapatens, like all other plants, is able to use light as a sole source of energy. Greenovation makes use of this capacity by culturing the moss in Photobioreactors, which have high-performance light sources installed outside the culture tank. In contrast to heterotrophic systems like microbial or mammalian cell cultures, requiring much more complex media, this photoautotrophic process is based on rather simple, purely mineral media. The obvious economic benefit is not only due to media cost itself, but is also reflected downstream in high initial purity and generic purification paths.

Being a higher eukaryote, Physcomitrella possesses the enzymatic machinery to carry out complex posttranslational modifications. These enzymes, including e.g chaperones and glycosyl-transferases are lined up along the so called secretory pathway, which is the standard route used for recombinant protein production by BryoTechnology.

As a result, bryotechnological products are fully N-glycosylated with an N-glycan-structure holding some crucial benefits:
Aside from Glycoengineering, moss N-glycans are per se free of core a-1,6-fucose, a carbohydrate-structure which is typically present on N-glycans of mammalian-cell derived proteins. The absence of a-1,6-fucose has been proven to drastically increase the efficacy of IgG-products by enhancing ADCC (antibody-dependant cellular cytotoxicity). Mechanistically, this effect is due to increased CD16-binding and has dramatic potential in oncology applications.