Bryotechnology:
known procedures – better products

Full range of contract development and manufacturing

Greenovation offers the full range of contract development and manufacturing from small-scale feasibility studies to validated clinical- and commercial-scale production processes. This enables our clients not only to test potential protein candidates quickly, but also to receive drug substance for clinical trials and the market.

Moss-based biopharmaceutical production process
Moss-based biopharmaceutical production process
Strain development

Strain development

BryoMaster® enables development of stable production strains cultivated in a photoautotrophic fermentation process for production of large scale batches of pharmaceutical proteins such as LSD-enzymes, ADCC-enhanced mAbs, and immunotoxins.

  • Protoplast transformation
  • PEG-mediated protocol
  • Linear DNA, no vector backbone
  • Regeneration of entire moss plants
  • Product-based screening
  • Genetically stable
  • Eucaryotic PTMs
  • Customized platforms
  • Free of animal components
  • Antibiotic-free
Cell banking

Cell banking

Greenovation offers master cell banking with cryo storage (-196°C) using disposable equipment.

OUR CELL BANKS ARE TESTED FOR

  • Identity
  • Purity
  • Homogeneity
  • Stability
Process development and upscaling

Process development and upscaling

UPSTREAM

  • Single use biorecators
  • Product secretion
  • Suspension culture
  • Batch-, perfusion or continous processes
  • Batch-to-batch stability
  • Scale-down, multiparallel fermentation facility for design of experiment (DOE) setups

DOWNSTREAM

  • Generic downstream, comparable to e.g. CHO
  • Chromatography-based methods
  • Low HCP-background
  • Proprietary HCP-ELISA
Contract manufacturing

Contract manufacturing

Apply our clinically proven BryoTechnology for development & cGMP-compliant production of your proteins. BryoTechnology offers a wide range of advantages for biopharmaceutical development and production processes.

BRYOMASTER® DELIVERS BETTER PRODUCTS

  • Genetically stable strains
  • Glycodesign
  • Batch to batch stability
  • Animal component free
  • Antibiotic-free
  • Customized platforms
Proof-of-concept studies

PoC studies

BryoSpeed® offers rapid production of recombinant products with full posttranslational modification. By applying a matrix of different parameters, you get the answers you need in one step.

MORE INFORMATION IN JUST ONE STEP

  • Multi-parallell screening of proteins by using a matrix of different parameters
  • Highly controllable conditions (media, light, temperature, CO2, and shaking rate)
  • Short delivery time
Outstanding product quality

Outstanding product quality

Exploiting the unique advantages of the moss Physcomitrella patens, BryoTechnology is a production process based on single-use technology and chemically-defined fermentation medium free of antibiotics and animal-derived components. The process is cGMP-compliant, highly scalable and allows for straightforward tech transfer to any given manufacturing plant.

YOUR ADVANTAGES

  • Glyco-designed products
  • High levels of batch-to-batch homogeneity
  • Customized production strains
  • Free of animal components
  • Antibiotic-free
Typical products: complex, difficult-to-express, posttranslationally modified proteins

Wide range of proteins expressed

BryoMaster® offers an improved solution targeting complex, difficult-to-express, posttranslationally modified proteins.

SUCCESSFULLY PRODUCED (examples)

  • IgG
  • IgG-toxin fusion
  • monomeric IgA
  • Glucocerebrosidase
  • alpha-Galactosidase A
  • acid alpha-Glucosidase
  • Complement factor H
  • VEGF
  • FGF7 (KGF)
  • EGF
  • ...

Be part of it

BryoTechnology offers a wide range of advantages for biopharmaceutical development and production processes. Our broad IP portfolio covers plant production processes, N-glycosylation techniques, proprietary drug candidates, and mechanisms-of-action. We offer contract manufacturing and licensing models.

Contact

Manon Kirstein
Head of Business Development
Phone + 49 761 470 99 0
bd@greenovation.com